Carbon catabolism of Aspergillus niger grown on sugar beet pulp

Benoit Gelber Isabelle1, Zhou Miaomiao1 , Vivas Duarte E Alexandra1, Downes Damien J.2, Todd Richard B.2, Post Harm3, Heck Albert J. R.3, Altelaar A. F. Maarten3, de Vries Ronald P.1

Author address: 

(1) Fungal Physiology CBS-KNAW Fungal Biodiversity Centre, Utrecht, THE NETHERLANDS; (2) Department of Plant Pathology, Kansas State University, Manhattan, KS, USA; (3) Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, THE NETHERLANDS

Abstract: 

Degradation of plant biomass to fermentable sugars is of critical importance for the use of plant materials for biofuels and in bio based economy. Filamentous fungi are ubiquitous organisms and major plant biomass degraders. Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. During growth, the mycelium encounters heterogeneous carbon sources. We assessed whether substrate heterogeneity is a major determinant of spatial gene expression in colonies of Aspergillus niger. We analyzed whole genome gene expression in five concentric zones of colonies utilizing sugar beet pulp. Growth, protein production and secretion occurred throughout the colony. Analysis of genes involved in carbon catabolism, genes encoding plant biomass degrading enzymes and their regulatory aspect will be presented.

2016

abstract No: 

35

Full conference title: 

The Thirteenth International Aspergillus Meeting, EUROSITES La Chapelle, PARIS, FRANCE
    • Asperfest 13 (2016)