Breath Volatile Organic Compound (VOC) Profiles for the Diagnosis of Invasive Aspergillosis (IA)

Ref ID: 18635

Author:

S. Koo, MD – Physician1,2, H. R. Thomas, AB – Res Asst 1, P. Rearden, PhD – Staff 3, J. Comolli, PhD – Staff 3, L. R. Baden, MD – Physician 1,2, F. M. Marty, MD – Physician

Author address:

1Brigham and Women’s Hosp., Boston, MA, 2Harvard Med. Sch., Boston, MA, 3Draper Lab., Cambridge, MA.

Full conference title:

52nd Annual ICAAC

Date: 9 September 2014

Abstract:

Background: There is a need for improved IA diagnostics. We studied detection of fungal VOCs in human breath as a novel IA diagnostic approach.
Methods: 5 A. fumigatus (AF), 3 A. terreus (AT), and 3 A. calidoustus (AC) species (type and invasive clinical strains) were cultured at 37°C in conditions promoting hyphal growth and suppressing sporulation. VOCs from in vitro cultures, breath of patients with and without IA, and ambient air controls were concentrated onto thermal desorption traps designed to retain VOCs of diverse size and polarity. Using gas chromatography-mass spectrometry, we defined VOC profiles of these Aspergillus species and patient breath samples. Results: We identified a characteristic AF VOC profile containing several terpenes (Figure); chamigrene, acoradien, elixene, α -santelene, and α -elemene in AT; 9-decene-2-one, 2-butanone, and β -sequiphellandrene in AC. These VOCs first appeared at 24h and intensified over 72h in vitro. AF had distinct VOC responses to antifungals: a 17-fold farnesene increase with 24h of micafungin and complete VOC profile attenuation with 24h of voriconazole exposure. 3 patients with proven or probable IA had breath farnesene indicators that were absent in the breath of 5 patients without IA and concurrent ambient air samples (Figure). In initial explorations, A. flavus, A. niger, R. oryzae, M. velutinosus, and F. solani have distinct VOC profiles. Conclusions: Common pathogenic molds have species-specific VOC profiles in vitro and in vivo. These unique VOC profiles are promising targets for IA diagnosis.

Abstract Number: M-1060

Conference Year: 2012

Link to conference website: NULL

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