Azole drug species-dependent responses of the transcription factor AtrR in Aspergilli

Ref ID: 18342

Author:

Ayumi Ohba , Kiminori Shimizu , Takahiro Shintani ,
Susumu Kawamoto , Katsuya Gomi

Author address:

Graduate School of Agricultural Science, Tohoku university, Japan Medical Mycology Research Center, Chiba
University, Japan

Full conference title:

Asperfest 8

Abstract:

It has hitherto been shown that overexpression of a transcription factor gene (atrR) resulted in increased azole drug resistance and also in upregulation
of gene expression of PDR-type ABC transporters in Aspergillus oryzae. In contrast, deletion of atrR led to downregulation of ABC transporter gene
expression and consequently resulted in significant increase in azole drug susceptibility. Previously, we revealed that expression of atrR and three PDRtype ABC transporter genes (atrA, atrF, and atrG) are upregulated by miconazole in A. oryzae wild-type strain. In this study, we investigated the
expression profiles of atrR and the PDR-type ABC transporter genes in the presence of other azole drugs. The atrR and PDR-type ABC transporter genes
(at least atrA, atrF, and atrG) were upregulated by clotrimazole, but not by itraconazole, ketoconazole, or fluconazole, although deletion of atrR resulted
in significant increase in itraconazole and fluconazole susceptibility. This suggested that the transcription factor AtrR shows different responses dependent
on azole drug species. To identify the target genes regulated by AtrR in the presence of different azole drug, we performed DNA microarray analyses of
wild type and atrR disruptant, which will be presented. In addition, expression profiles of the atrR ortholog and ABC transporter genes in several azole
drugs were also examined in human pathogenic fungus, A. fumigatus.

Abstract Number: 20)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster