Asymmetric RNA localization in Aspergillus fumigatus.

Ref ID: 18351

Author:

Mara Couto-Rodrí­guez, Susan Cowden, Ken Oda and Michelle Momany.

Author address:

Plant Biology
Department, University of Georgia, Athens, Georgia 30602.

Full conference title:

Asperfest 8

Abstract:

Filamentous fungi, such as Aspergillus fumigatus, are characterized by a highly polarized growth that occurs mainly by hyphal tip extension. Fungal tip
growth involves many processes such as cell wall synthesis, vesicle transport, exocytosis and endocytosis. Even though there has been a reasonable amount
of progress understanding these processes in the past few years, the exact mechanisms that regulate establishment and maintenance of polarity are not
completely understood. Recent studies of highly polar cells from Drosophila melanoganster, Candida albicans, Ustilago maydis and others have
demonstrated that RNA localization is used to restrict translation spatially and temporally. Consequently, we investigated asymmetric RNA localization
in A. fumigatus . Laser microcapture combined with 454 sequencing done in our lab identified many of transcripts that appeared to be asymmetrically
localized in polar A. fumigatus cells. In order to validate the level of asymmetry detected by 454 sequencing we performed Fluorescent in situ
hybridization (FISH) . Transcripts from tip, base and conidia that showed the greatest asymmetry and the highest expression levels were chosen to
synthesize digoxigenin labeled dsDNA probes for in vivo detection in A. fumigatus germlings. FISH experiments confirmed that many individual mRNA’s
are differentially localized to tip, base and conidium.

Abstract Number: 29)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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