Aspergillus immunoblot: a new diagnostic tool

Ref ID: 19232

Author:

A. Oliva*, M. Cornet, P. Flori, C. Hennequin, H. Pelloux, R. Piarroux, S. Ranque

Author address:

Marseille, Grenoble, St. Etienne, Paris, FR

Full conference title:

23rd European Congress of Clinical Microbiology and
Infectious Diseases

Date: 27 April 2014

Abstract:

Objectives: Specific antibodies detection is key to diagnose aspergillosis in immunocompetent patients. Although not standardized, immunoprecipitin detection (IPD) is the current gold standard. The aim of this study was to evaluate the utility of a new commercial immunoblot (WB) kit (Aspergillus WB IgG – LDBio Diagnostics, Lyon, France) as a diagnostic tool for chronic aspergillosis.
Methods: Sera from two groups of patients with proven, suspected or possible chronic aspergillosis (group 1) and cystic fibrosis patients with either allergic bronchopulmonary aspergillosis or Aspergillus colonization (group 2) were collected in the Parasitology and Mycology Laboratories of four French University Hospitals (Grenoble, Marseille, Saint Etienne and Saint Antoine, Paris). Blood donors’ sera (group 3) were used as healthy controls. Excepted for group 3, IPD has been performed in each laboratory as part of the patients’ routine diagnostic work-up. WB was performed on all sera using the Aspergillus WB IgG kit (LDBio Diagnostics, Lyon, France) according to the manufacturer’s recommendations.
Preliminary Results: To date, 249 sera from aspergillosis cases (respectively 176 and 73 sera for group 1 and 2) and 213 healthy control sera were analyzed. 99% of the positive sera displayed at least a three specific bands WB pattern, as described in Aspergillus WB IgG kit instructions. WB specificity, as calculated over group 3, was at 96%. Sensitivity ranged from 67% to 100%, depending on the patients’ diagnosis. It increased with aspergillosis categorization level; i.e., for group 1, the Aspergillus WB gave the best results in proven aspergillosis cases (Table 1). Overall, the results showed that the WB was at least as sensitive as the currently used IPD assays (Table 1). Finally, it was notified that the use and the interpretation were easier for WB than for IPD.
Conclusion: These promising preliminary results highlight the interest of this novel immunoblot assay for the diagnosis of aspergillosis in immunocompetent patients. Further studies are warranted to confirm its performance.

Abstract Number: P1738

Conference Year: 2013

Link to conference website: http://registration.akm.ch/einsicht.php?XNABSTRACT_ID=165309&XNSPRACHE_ID=2&XNKONGRESS_ID=180&XNMASK

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster