Aspergillus clavatus as a potential enzyme source to use in biomass degradation to produce second8208;generation ethanol: Cloning and expression of hemicelulases after secretomic analysis using different pre8208;treatment sugar cane bagasse<

Ref ID: 18523

Author:

Rosymar Coutinho de Lucas,
Simone Carvalho Peixoto Nogueira,
André Ricardo de Lima Damásio,
Fábio Marcio
Squina,
Rolf Alexander Prade,
Héctor Francisco Terenzi,
João Atí­lio Jorge,
Maria de Lourdes Teixeira de
Moraes Polizeli,
Fernando Segat

Author address:

USP/RP-Sao Paulo University,
CTBE-National Laboratory of Science and Technology of Bioethanol,  
OSU- 
Oklahoma State University

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Aspergillus clavatus is a cosmopolitan fungus that had its genome sequenced by The Institute for Genomic
Research. A detailed comparative genomic analysis using a large number of fungi database showed that this
microorganism is a potential enzymes producer. In addition, there are few studies about this fungus. The plant cell
wall polysaccharides are being considered as source of renewable energy such as biofuels owing to the fact that
they are the most abundant reserves of carbon. It can be conveniently divided into three groups namely cellulose,
pectin and hemicellulose, with hemicellulose being the second most abundant biopolymer component of plant cell
wall and composed by xylan, arabinan, mannan, glucomannan, galactomannan, and glucogalactomannan. Due to
its complexity a large set of enzymes are necessary to degrade the plant cell wall. In order to study potential
enzymes directly involved in degradation of the most abundant brazilian biomass we report in this work, the
comparative analysis of the A. clavatus secreted in 5 different pre8208;treatment sugar cane bagasse using glucose as
the control, cloning and expression of hemicellulases. The proteomic analyses have identified 135 different
proteins where 2% of those are enzymes related with biomass degradation. The relative difference reflect the
necessity to use specific enzymatic pool to degrade different pre8208;treated sugar cane bagasse, because of diference
in the sugar compositions. In addition, to perform the heterologous protein expression of A. clavatus hemicellulase
we used our Aspergillus nidulans expression system and the recombinant enzymes were highly expressed and
secreted to the culture medium.

Abstract Number: PR8.26

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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