Asexual development is regulated by histone H3 acetylation in Aspergillus nidulans.

Ref ID: 18343

Author:

David Cánovas*, Yazmid Reyes-Domí­nguez#, Ana T.
Marcos*, Ulrich Güldener‡, and Joseph Strauss

Author address:

*Departamento de Genética, Facultad de Biologí­a, Universidad de Sevilla, Spain, davidc@us.es; #Fungal
Genetics and Genomics Unit, AIT-Austrian Institute of Technology and BOKU University, Vienna, Austria. “¡Institute of Bioinformatics and Systems
Bi

Full conference title:

Asperfest 8

Abstract:

The ascomycete Aspergillus nidulans is a model organism to study fungal development. The expression of the brlA gene triggers the formation of the
developmental structures, the conidiophores. The expression of brlA is regulated by a number of upstream regulators, including FluG, FlbA-E. In addition
to these regulators, we have found that the product of gcnE is necessary for the expression of the brlA gene. GcnE is a homolog of yeast GCN5p, the
catalytic subunit of the conserved SAGA/ADA complexes responsible for the majority of lysine acetylation in histone H3 (H3ac) and subsequent
transcription-related chromatin remodelling. In A. nidulans, deletion of gcnE results in a severe defect of asexual development. In this study, we compared
wild type and the gcnE) mutant by transcriptome analysis and chromatin modification assays. Microarray analysis revealed major effects on the expression
of genes involved in primary and secondary metabolism as well as in development. Consistently, the expression of brlA is dramatically reduced and some
of the upstream regulators are deregulated. Chromatin immunoprecipitation assays revealed an altered pattern of H3ac in promoters of the conidiation
regulators, suggesting that H3 acetylation carried out by GcnE is required for the accurate regulation of conidiation.

Abstract Number: 21)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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