ApsB, a component of microtubule8208;organizing centres resides in microbodies

Ref ID: 18460

Author:

Andreas Herr, Ying Zhang, Nadine Zekert, Reinhard Fischer 

Author address:

Karlsruhe Institute of Technology, Germany

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

In Aspergillus nidulans septum8208;associated microtubule8208;organizing centres (sMTOCs) together with spindle pole
bodies (SPBs) polymerize cytoplasmic microtubules. Previously, we identified a novel MTOC8208;associated protein,
ApsB (Schizosaccharomyces pombe mto1), whose absence affected MT formation from sMTOCs more than from
SPBs, suggesting that the two protein complexes are organized differently (Suelmann et al., 1998). sMTOCs share
at least two further components, gamma8208;tubulin and GcpC (S. pombe Alp6) with SPBs, and ApsB interacts
physically with gamma8208;tubulin. Surprisingly, ApsB contains a peroxisomal targeting sequence, PTS2, which could
be replaced by a PTS1 motif at the C8208;terminus of ApsB (Zekert et al., 2010). Here, we show that fractions of ApsB
and gamma8208;tubulin indeed reside in microbodies. Both proteins co8208;fractionated with microbodies in sucrose
gradients. Protein protection assays revealed that ApsB and gamma8208;tubulin reside inside microbodies suggesting a
novel role for these organelles in microtubule organization. As a further proof for such a function, we created a
deletion mutant of pexC. PexC is an essential protein for peroxisomal biogenesis (Heiland & Erdmann, 2005). In
agreement with our hypothesis, the 8710;pexC strain partially phenocopied the 916;apsB mutation.
Heiland, I. & Erdmann, R. (2005). The FEBS journal 272, 23628208;2372.
Suelmann, R., Sievers, N., Galetzka, D., Robertson, L., Timberlake, W. E. & Fischer, R. (1998).  
Molecular microbiology 30, 8318208;842.
Zekert, N., Veith, D. & Fischer, R. (2010). Eukaryot Cell 9, 7958208;805. 

Abstract Number: PR1.43

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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