Antifungal susceptibility of clinical Cryptococcus neoformans/C. gattii complex isolates in Korea

Ref ID: 17751


Eunjung Kang, Hye Seung, Lee, Han Gyeol Kim, Hyun Kyung Kim, Seung-Kwan Lim, Young Hwa Choi.

Author address:

(Suwon, KR)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases


Objectives: Cryptococcosis is an opportunistic infection caused by two
species of the genus Cryptococcus neoformans and Cryptococcus gattii.
We previously reported the molecular epidemiological analysis of 78
clinical strains of the C. neoformans/C. gattii species complex, but
antifungal susceptibility test was not taken. The Purpose of this study is
to demonstrate the pattern of antifungal susceptibility of Cryptococcus
neoformans/C. gattii Complex of South Korea.
Methods: Strains The 78 strains were isolated from the clinical
specimens recovered from cryptococcosis patients who had been
hospitalized between 1999 and 2008 in seven medical centers in South
Antifungal susceptibility 1 Microdilution test
Microdilution test was carried out according to the Clinical Laboratory
Standard Institute M27-A3 document. Amphotericin-B, voriconazole
and fluconazole were tested against all isolates twice. C. parapsilosis
ATCC 22019 was included as quality control.
2 VITEK-2 system
VITEK-2 system was performed according to the manufacturer’s
Statistics: Statistical analysis was performed using the program SPSS
version 18.0 software, and p-value < 0.05 was used to define significance. We compared the MIC of fluconazole in microdilution test according to the time period divided by 1999-2000 and 2001- 2008. The Mann-Whitney test was used for comparison of MIC according to the time period. Results: Among 78 strains, 75 strains (96%) were C. neoformas serotype A and three strains (4%) were C. gattii, serotype B. The antifungal susceptibility test results shows in Table 1. There is no resistant strain to amphotericin-B and voriconazole. Two strains are susceptible dose dependent for fluconazole in broth microdilution test. One strain in 73 strains appears to be resistant for flucytosine in VITEK-2 system. Eleven strains were recovered in 1999 to 2000 and 67 strains were in 2001 to 2008. Geometric means were 5.84 and 4.54 respectively. The difference of MIC between the two time periods was not statistically significant. (p = 0.698)

Abstract Number: P817

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Conference Year: 2012

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