Antifungal activity of Iranian honeybee with different botanical sources against Candida, Aspergillus species and Trichophyton rubrum

Ref ID: 19374

Author:

F. Katiraee and R. Mahmoudi

Author address:

Faculty of Veterinary Medicine, University of Tabriz, Iran

Full conference title:

6th Trends in Medical Mycology 2013

Date: 11 October 2014

Abstract:

Objectives Due to limitation for choice suitable antifungals, search
for new antifungals is necessary and stimulates research on new che-
motherapeutic agents in natural products. The current study was
carried out to assess the antifungal activity of different Iranian honey
from different botanical origin against some important fungi, as well
as to determine activity against azoles-resistant Candida species.
Methods Seven types of honey samples include Thymus vulgaris,
Alfalfa, Citrus, Zizyphus,Astragalus, Chamaemelum nobile and multi
flora honey were collected. Candida yeast strains included Candida albi-
cans, C. dubliniensis, C. parapsilosis, C. tropicalis, C. glabrata, C. Krusei,
C. kefyr, Aspergillus niger, Aspergillus fumigates, Aspergillus flavus, and
Trichophyton rubrum was used in this study. Clinical Candida strains
identified and were confirmed by PCR-RFLP. MICs microdilution
broth method was used for antifungal activity of honeys based on
the CLSI, M27-A2. Stocks and dilutions of honeys and fluconazole
(for Candida species) were prepared in sterile distilled water. Final
drug concentrations in the microdilution plates ranged from
64-0.125 lg/ml for fluconazole and from 80-10% (v/v) for all of the
honeys. The microdilution plates were prepared by using the RPMI
1640 broth medium with MOPS & glutamine and without sodium
bicarbonate. Yeast suspensions were prepared after vortexing and
adjusting to a 0.5 Mc Farland standard transmittance at a wave-
length of 530 nm. The final inoculum yielded was of 0.5 9 103
2.5 9 103 cells/ml. MICs were visually determined at 24 h of incu-
bation at 35°C, and were observed for the presence or absence of
growth.
Results All of the honey samples had antifungal activity against the
tested microorganisms, ranged from 15-60% (v/v).The results
revealed variations in the antifungal activity of the different honey
samples. Alfalfa & Thymus honeys were significantly more effective
and Zizyphus & Chamaemelum honeys exhibited lowest antifungal
activity. The differences were considerable, the order of activity in
terms of overall activity against all; Alfalfa > Thymus > Astragalus >
multifloral > Citrus> Chamaemelum > Zizyphus. Trichophyton rubrum
was the most and C. krusei was the least susceptible of the fungal
species. Significant differences were determined between susceptibili-
ties of T. rubrum and Candida species to Multiflora, Thymus, Alfalfa,
Citrus, Zizyphus and Chamaemelum honeys and not for Astragalus. No
statistically significant differences of susceptibility to honey samples,
between fluconazole resistance and susceptible Candida species were
determined.
Conclusion Honey could be applied in the therapy of fungal infec-
tions includes oral, vaginal and cutaneous candidiasis, dermatomyco-
sis and dermatophytosis. On the other hand, the fungal infections
have emerged as a major cause of disease and mortality in immuno-
compromised patients. Accordingly, emergence of fungi resistant to
antifungal agents, side effects and limited spectrum has created a
need for antifungal agents. With using particular originated honeys,
researchers and practitioners could select the honey with defined ori-
gin source for different types of fungal infection, superficial mycoses,
and resistant Candida species and with a high probability of successful
results.

Abstract Number: p006

Conference Year: 2013

Link to conference website: NULL

New link: NULL

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