Purpose: The filamentous fungus Aspergillus fumigatus is capable of causing a range of diseases in immunocompromised patients and is responsible for up to 3% of all hospital-based deaths in the EU. Caspofungin is a recently introduced antifungal compound that has good efficacy against fungi with minimal side effects. The aim of the work presented here was to characterise the response of A. fumigatus to caspofungin over a range of environmental conditions.
Methods: Caspofungin retards fungal growth but the response to this drug is influenced by a variety of environmental factors (e.g. pH, serum, glucose). Exposure of A. fumigatus hyphae to this drug leads to the release of amino acids and gliotoxin. A. fumigatus hyphae exposed to caspofungin demonstrated an increase in the expression of proteins associated with an oxidative stress response (e.g. catalase, antibiotic response protein) and in the activity of selected enzymes (e.g. catalase, glutathione reductase and superoxide dismutase).
Results: The results presented here demonstrate that a variety of environmental conditions alter the susceptibility of A. fumigatus to caspofungin.
Conclusion: A variety of environmental conditions may alter the activity of caspofungin but this work also shows that environmental factors such as pH and serum or glucose content alter the susceptibility of A. fumigatus to this drug.
Full conference title:
- AAA 8th (2018)