Exome sequencing of individuals with aspergillosis demonstrates genetic variants that may impact on the structure/function of the respiratory epithelium and replication of these mutations, using CRISPR/Cas9 technology, results in morphological epithelial change. However, the transcriptional and translational response of the bronchial epithelium to Aspergillus fumigatus is poorly defined. Using a 16HBE cell line model, RNA-Seq and flow cytometry, we describe transcriptional and secretory epithelial responses to A.
Full conference title:
- ERS 26th (2016)