A proteome reference map of Aspergillus nidulans and new putative targets of the AnCF complex

Ref ID: 18297

Author:

K. Tuppatsch, O. Kniemeyer, P. Hortschansky,

Author address:

A. A. Brakhage Department of Molecular and Applied Microbiology, Leibniz Institute
for Natural Product Research and Infection Biology (HKI), Jena, Germany katja.tuppatsch@hki-jena.de

Full conference title:

Asperfest 9

Abstract:

The mould Aspergillus nidulans is a well suited model organisms for filamentous fungi and is closely related to many Aspergillus
species of industrial and medical interest. With the completion and publication of the A. nidulans genome it is feasible to study gene
expression and protein production on a global scale. A variety of transcriptome studies have been already carried out for A. nidulans.
By contrast, only little information is available about the dynamic changes of the proteome of A. nidulans upon environmental
changes, stress conditions or genetic modifications. Furthermore, no proteome map for has been published so far. For this reason, we
established the first 2-D reference map for the intracellular protein fraction of A. nidulans strain TNO2A7. After 2D-gel
electrophoretic separation, visualisation of proteins by Coomassie staining and image analysis with Delta 2D, 435 spots representing
364 different proteins were identified by MALDI-TOF-MS/MS analysis. Quantitative proteomic analysis of a hapC deletion mutant
revealed many proteins with difference in abundance in comparison to the wild type. Two proteins, a conserved hypothetical protein
and a guanine nucleotide dissociation inhibitor, were found to be putative, so far uncharacterised targets of the AnCF complex.
Knock-out and double knock-out strains of the corresponding genes are in progress.

Abstract Number: 48)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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