A NsdD-dependent novel helix-loop-helix transcription factor is necessary for the conidiation of Aspergillus nidulans and Aspergillus fumigatus.

Ref ID: 18346

Author:

Kap-Hoon Han1, Seul-A Gu, Jae Sin Park, Lee Han Kim, Dong-Soon Oh1, and Dong Min Han

Author address:

Division of Life Science, Wonkwang
University, Iksan, 570-749, Korea 1Department of Pharmaceutical Engineering, Woosuk University, Samrye, 565-701, Korea

Full conference title:

Asperfest 8

Abstract:

The nsdD gene encodes a GATA type transcription factor, which controls sexual development positively in A. nidulans. According to the transcription
profile analysis using WT vs. nsdD deletion mutant, more than hundred genes were expressed by NsdD-dependent manner during various stages of life
cycle. One of them, named ndeA (nsdD-dependent expression), which encodes a conserved protein carrying helix loop helix (HLH) domain, showed the
preferential development of cleistothecia even under the various stress conditions, when deleted. Overexpression of the gene under niiA promoter resulted
in the failure of cleistothecia formation, suggesting that the gene controls sexual development negatively or positively controls conidiation. Northern
analysis revealed that the gene was transcribed at early stages of both sexual and asexual sporultion. The mRNA levels were increased in nsdD or veA
deletion mutant at same stages, indicating that the gene was repressed by those regulators during early development stages. In Aspergillus fumigatus,
knock-out of the homologous AfndeA gene resulted in aconidiation phenotype, indicating that this gene plays an important role in conidiation process
as well as sexual development.

Abstract Number: 24)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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