A Molecular genetic analysis of the AreA8208;NmrA interaction in Aspergillus nidulans

Ref ID: 18509

Author:

Trent Woodward, Meryl Davis, Alex Andrianopoulos

Author address:

Department of Genetics, University of Melbourne,  Parkville, Australia.

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The nitrogen metabolite repression system of Aspergillus nidulans allows the expression of genes required for the
utilisation of non8208;preferred nitrogen sources to occur only in the absence of the preferred ammonium or
glutamine. The transcriptional activation ability of the primary positive regulator of this system, AreA, is modulated
through many processes including differential areA mRNA stability, regulation of nuclear import/export and
competitive binding with the negative acting AreB. AreA function is also modulated by interaction with the co8208;
repressor NmrA and co8208;activator TamA. The co8208;repressor NmrA is able to bind to the GATA Zinc8208;Finger DNA
binding region of AreA, but in vivo experiments have shown that this binding is not preferential to AreA binding to
GATA containing DNA, so the exact mechanism with which NmrA represses the activity of AreA is unknown.
This study aims to investigate this interaction with and repression of AreA through a mutagenic approach. Since
overexpression of nmrA can prevent the activity of AreA, resulting in an inability to grow on non8208;preferred
nitrogen sources, a screen has been set up to obtain mutants that are insensitive to NmrA activity. The sequence
changes in these mutants and the predicted effects on NmrA structure have been determined.

Abstract Number: PR7.41

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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