Author:

Chang YC, Tsai HF, Karos M, Kwon-Chung KJ.

Date: 9 September 2004

Abstract:

Aspergillus fumigatus grows optimally from 37 to 42
C but can grow at temperatures up to 55
C. To study the genetic basis of thermotolerance and its role in virulence of A. fumigatus, temperature sensitive mutants were isolated. One of the mutants that grew at 42
C but not at 48
C was complemented and the gene, THTA, was identi?ed. Deletion of THTA showed the same temperature sensitivity as the original mutant. THTA encodes a putative protein of 141kDa with unknown function and the HA-tagged ThtAp accumulated to similar levels in cultures grown at either 37 or 48
C. Southern blot analysis and database searches revealed the presence of THTA-related sequences in several other ascomycetous fungi. No di?erence in virulence was observed between the DthtAand wild-type strains. Thus, THTA is essential for growth of A. fumigatus at high temperatures but does not contribute to the pathogenicity of the species.

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