The nsdC Gene Encoding a Putative C2H2-type Transcription Factor is a Key Activator of Sexual Development in Aspergillus nidulans
Author:
Kim HR, Chae KS, Han KH, Han DM
Date: 4 June 2009
Abstract:
The formation of Aspergillus nidulans fruiting body is affected by a number of genetic and environmental factors. Here, the nsdC (never in sexual development) gene, encoding a putative transcription factor carrying a novel type of zinc finger DNA binding domain consisting of two C2H2s and a C2HC motif that are highly conserved in most fungi but not in plants or animals, was investigated. Two distinct transcripts of 2.6 kb and 3.0 kb were generated from nsdC. The 2.6 kb mRNA accumulated differentially in various stages of growth and development, while the level of the 3.0 kb mRNA remained relatively constant throughout the life cycle. While the deletion of nsdC resulted in the complete loss of fruiting body formation under all conditions favoring sexual development, over-expression of nsdC not only enhanced formation of fruiting bodies (cleistothecia) but also overcame inhibitory effects of certain stresses on cleistothecial development, implying that NsdC is a key positive regulator of sexual development. Deletion of nsdC also retarded vegetative growth and hyper-active asexual sporulation, suggesting that NsdC is not only necessary for sexual development but also regulating asexual sporulation negatively. Over-expression of veA or nsdD does not rescue the failure of fruiting body formation caused by nsdC deletion. Furthermore, nsdC expression is not affected by either VeA or NsdD, and vice versa, indicating that NsdC regulates sexual development independently of VeA or NsdD.
Download the full article (Disclaimer)
This manuscript library of ~16,000 articles (1729-2024) related to Aspergillus and aspergillosis is intended for individual study only, and is provided as contribution to global understanding of the topic. Please refer to the publisher’s guidance about any other usage.