Author:

Reyes G, Romans A, Nguyen CK, May GS

Date: 2 October 2006

Abstract:

The genome of Aspergillus fumigatus has four genes that encode mitogen-activated protein kinases (MAPKs), sakA/hogA, mpkA, mpkB, and mpkC. The functions of the MpkB and MpkC MAPKs are unknown in A. fumigatus or the closely related and genetically amenable species Aspergillus nidulans. mpkC deletion mutants were made in A. fumigatus and their phenotypes characterized. The mpkC deletion mutants were viable, had normal conidial germination and hyphal growth on minimal or complete media. This is in contrast to deletion mutants for the closely related MAPK gene sakA/hogA that we previously reported had a nitrogen source dependent germination phenotype. Similarly, growth of the mpkC deletion mutants was wild type on high osmolarity medium. Consistent with these two MAP kinase genes regulating different cellular responses, we determined that the mpkC deletion mutants were unable to grow on minimal medium with sorbitol or mannitol as sole carbon source. This result implicates MpkC signaling in carbon source utilization. Changes in messenger RNA levels for sakA and mpkC were measured in response to hypertonic stress, oxidative stress and on shift from glucose to sorbitol to determine if there was overlap in SakA and MpkC signaling pathways. These studies demonstrated that SakA and MpkC dependent patterns of changes in mRNA levels are distinct and have minimal overlap in response to these environmental stresses

Link to DOI

Download the full article (Disclaimer)

This manuscript library of ~16,000 articles (1729-2024) related to Aspergillus and aspergillosis is intended for individual study only, and is provided as contribution to global understanding of the topic. Please refer to the publisher’s guidance about any other usage.

I understand (click to accept and view download link)

---