The asexual pathogen Aspergillus fumigatus expresses functional determinants of Aspergillus nidulans sexual development

Author:

Große V, Krappmann S

Date: 22 September 2008

Abstract:

The major fungal pathogen of humans, Aspergillus fumigatus, lacks a defined sexual cycle although the presence of genes encoding putative mating type idiomorphs and regulators of Aspergillus sexual development heightens the potential for cryptic sexuality in this deuteromycete. To test the functionality of these genetic determinants we transferred the alpha box-encoding mat1-1 idiomorph from an A. fumigatus isolate to the homothallic fertile species Aspergillus nidulans. Abundant formation of fruiting bodies (cleistothecia) containing viable ascospores establishes functionality of this mating type gene product in the transgenic strain. Using a similar approach we also established that the conserved transcriptional regulator from A. fumigatus, the nsdD gene product, can act as a functional, positively-acting factor for A. nidulans cleistothecia development; moreover, high level expression of NsdD in the endogenous host A. fumigatus profoundly alters hyphal development by triggering the formation of coiled hyphae. Our findings demonstrate that the presumably asexual pathogen A. fumigatus encodes functional regulators of mating and sexual development thereby potentiating the case for cryptic sexuality in this major fungal pathogen.

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