Author:

OLE FILTENBORG, JENS C. FRISVAD, AND JYTTE A. SVENDSEN

Date: 23 December 2005

Abstract:

A simple screening method for molds producing the intracellular mycotoxinsbrevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A,roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegninwas developed. After removing an agar plug from the mold culture, the myceliumon the plug is wetted with a drop of methanol-chloroform (1:2). By this treatmentthe intracellular mycotoxins are extracted within seconds and transferred directlyto a thin-layer chromatography plate by immediately placing the plug on the platewhile the mycelium is still wet. After removal of the plug, known thin-layerchromatographic procedures are carried out. The substrate (Czapek yeast autolysateagar) and growth conditions (25°C for 7 days) used by Penicillium taxonomistsproved suitable for the production of the mycotoxins investigated when 60known toxigenic isolates and 865 cultures isolated from foods and feedstuffs weretested with this screening method.

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