Author:

Gayakwad SR; Harne SD; Kalorey DR; Ingle VC.

Date: 4 April 2004

Abstract:

Out of 100 feed samples collected from different poultry farms located in the Nagpur region screened, 94 (94 percent) showed the presence of fungi with a predominance of mixed type of fungi in 76 (76 percent) feed samples. A total of 309 fungal isolates were recovered, of which Aspergillus spp. (53.33) were predominant followed by Penicillium spp. (17.47 percent), Fusarium spp. (5.82 percent), Mucor spp. (5.17 percent), Cladosporium spp. (3.88 percent), Curvularia spp. (2.58 percent), Rhizopus spp. (2.26 percent), Absidia spp. (2.26 percent), Syncephalosotrum spp. (1.29 percent) and Cunninghamela spp. (1.29 percent). Among the 171 Aspergillus isolates, 45.61, 28.65, and 8.65 per cent were found to be aflatoxigenic by thin layer chromatography (TLC), reverse yellow pigmentation on Czapek’s -dox- agar (CDA) and reverse blue fluorescence on coconut agar medium (CAM), respectively. Simultaneously, Kojic acid production was observed in Aspergillus spp. (54.38 percent) and Penicillium spp. (14.8 percent) by employing kojic acid test. Brilliant green yellow (BGY) pigmentation, visual and fluorescence methods were found to be simple and easy to perform for detection of kojic acid producing fungal strains.

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