Microbiological analysis of bottled water
Author:
Reddy PS
Date: 4 April 2004
Abstract:
ABSTRACT: We analyzed the microbiological quality of bottled water manufactured and marketed in Hyderabad. Ten brands were analyzed for presumptive coliform count by multiple tube test and confirmed E.coli count by Eijkman test. Bacterial, fungal and free-living parasite load were tested by membrane filtration test. One out of ten samples (10 percent) showed the presumptive coliform count to be 24 most probable number (MPN)/100ml, but was found to be negative when tested by Eijkman test for E.coli. In the membrane filtration test, 5 types of bacteria were grown from 10 samples with 3 samples showing more than 2 types of bacteria (30 percent). The variety of bacteria grown included Bacillus spp. from 5 Pseudomonas spp. from 4, Acinetobacter spp. from 2, Enterobacter spp. from 1, and Xanthomonas maltophilia from 1 sample. The mean colony count was 1.01 CFU/ml (0.02-3.7 CFU/ml). Fungi were isolated from 6/10 (60 percent) samples [Aspergillus spp-5, Candida spp-1; mean colony count 0.06 CFU/ml (0.05-11 CFU/ml)]. The lone sample with positive presumptive coliform count grew Enterobacter spp. with highest colony count (3.7 CFU/ml) along with Apergillus fumigatus (0.05 CFU/ml.) None of the samples showed the presence of free-living amoeba. The number and type of organisms recovered from the bottled water samples tested in this study were within the permissible levels prescribed for safe drinking water.
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