Measurement of voriconazole in serum and plasma
Author:
Langman LJ, Boakye-Agyeman F
Date: 8 November 2007
Abstract:
OBJECTIVES:: Voriconazole is an antifungal agent structurally related to fluconazole. While regular drug monitoring is not indicated in most patients, it may help guide dosing in patients with reduced hepatic/renal function, on concurrent therapy with drugs that affect CYP2C9, with altered CYP2C9 genotypes, or during adverse drug reactions. Here we describe an HPLC method for determination of voriconazole. !
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!METHODS:: Samples, calibrators and controls were extracted using a liquid/liquid extraction. Chromatographic separation achieved using gradient solvent delivery with detection at 254 nm with a run time of 10 min. Concentration was calculated by comparison of peak height ratio of the drug with that of internal standard (IS) against a standard curve. !
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!RESULTS:: The assay is linear from 0.29 to 57 mumol/L (0.1-20 mug/mL) and shows good linearity (y=0.98x+0.36, r(2)=0.9978). The assay has inter- and intra-day precisions of <10%. The stability of the drugs in specimens was tested for up to 7 days at room temperature, for 30 days frozen at -20 degrees C, and through 3 freeze-thaw cycles and was found to be stable under those conditions. !
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!CONCLUSIONS:: This describes a robust method for the determination of voriconazole in serum and plasma.
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