Long Term Preservation of Commercial Important Fungi in Glycerol at 4°C
Author:
Jai Shankar Paul, K.L. Tiwari and S.K. Jadhav
Date: 1 January 2015
Abstract:
Preservation of commercial and research important fungi for long time period is a very tedious job. Mostly cryopreservation technique in liquid nitrogen is used for long term preservation. In this work 15 different species of commercial and research important fungi are preserved at 4°C in different concentration of glycerol using two different methods (Slant culture and Slice cut method). In slant culture method at 50% of glycerol, 100 and 86.66% of fungi are viable upto 24 and 30 months of preservation, respectively. In slice cut culture method 100% of fungi are having regeneration capacity upto 24 months of preservation. This study help to preserve the fungi with easy and low cost for long term period at 4°C under refrigerator.
Key words: Concentration of glycerol, slant culture method, slice cut method, regeneration, preservation
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