Author:

ME García, JL Blanco & WP Kurup

Date: 4 April 2004

Abstract:

We have studied the immunochemical and biochemical differences in 12Aspergillus fumigatusstrains isolated from different sources. The enzymatic activityof all these strains were studied by a rapid enzyme detection method (APIZYM).One of 12 strains studied produced alkaline phosphatase, while twoproduced chymotrypsin, and three produced trypsin.By SDS-PAGE we studied proteins present in the antigen extracts from all 12strains. Several of the protein bands were unique and may be used to differentiatethe strains. One such protein is the 58 kDa band present in the mycelialextract and the 33 kDa in the culture filtrate.By crossed immunoelectrophoresis, differentiation of the strains isolated fromcystic fibrosis patients can be made based on a few specific precipitin arcs developedagainst anti- Aspergillusrabbit serum.

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