Author:

L Rebordinos, I Vallejo, M Santos, IG Collado, M Carbú & JM Cantoral

Date: 4 April 2004

Abstract:

Botrytis cinerea is a plant-pathogenic fungus that produces the disease knownas grey mould in a wide variety of agriculturally important hosts in many countries.Ten strains from different locations collected on different years have beenisolated and characterized by several methods (morphological, biochemical,genetical and molecular). Results showed that clear morphological differencesexist between strains, and showing a relationship between the presence of sclerotiaand pathogenicity. The conidial size and the nuclear number were highlyvariable between different strains. Pulsed-field gel electrophoresis showed a uniquekaryotype for each strain, highly polymorphic between strains and with anumber of bands ranging from 4 to 8.An efficient transformation system has been achieved through the plasmidpAMPF21, containing the region AMA1 of Aspergillus nidulans. Lastly, from agenomic library the gdhAgene has been cloned. This gene produces an RNAmof 1.7 Kb and complements the deficiency on glutamate dehydrogenase activityof A. nidulans.

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