Author:

M Tequida-Meneses, M Cortez-Rocha, EC Rosas-Burgos, S López-Sandoval y C Corrales-Maldonado

Date: 4 April 2004

Abstract:

Fungicidal activity of wild plants Larrea tridentata, Karwinskia humboldtiana,Ricinus communis, Eucalyptus globulus, Ambrosia ambrosioides, Nicotiana glauca,Ambrosia confertiflora, Datura discolor, Baccharis glutinosa, Proboscideaparviflora, Solanum rostratum, Jatropha cinerea, Salpianthus macrodonthus ySarcostemma cynanchoides was evaluated against the moulds speciesAspergillus flavus, Aspergillus niger, Penicillium chrysogenum, Penicilliumexpansum, Fusarium poae y Fusarium moniliforme moulds species. Alcoholicextracts 6% (w/v) were prepared using six grams of dried plant powders (leaves and stems) and alcohol (70% ethanol or 70% methanol). A spore suspension(1×106 ufc/ml) of each mould was prepared by adding saline solution (0.85%)and 0.1% tween 80. The Extracts were mixed with Czapeck yeast agar (CYA) at45-50 °C in 1:10 relation on Petri dishes. Triplicate Petri dishes of each treatmentand for each mould were centrally inoculated and three Petri dishes wereused without treatment as controls. The inoculated dishes and controls wereincubated at 25 ± 2 °C for eight days. The incubated dishes were examinatedeach 48 h and after the colony diameter (radial growth) was measured. Twomould species were controlled by L. tridentata, B. glutinosa and P. parviflora.Extracts of L. tridentata in methanol or ethanol at 41.5-100% inhibited all six speciesof moulds.

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