Author:

Paliwal A; Bir N; Raghuraman S; Reddy TLP; Sharma PU

Date: 4 April 2004

Abstract:

ABSTRACT: Oligonucleotide primers were synthesised based on the gene sequence of an 18 kDa allergen/antigen of A.fumigatus isolated from a pathogenic strain. Polymerase chain reaction (PCR) was carried out using the forward and reverse primers and genomic DNA of A.fumigatus, A.flavus and A.niger as template. This resulted in a PCR product of 480 bp with only A. fumigatus. The absence of PCR product in A. flavus and A. niger with the primers of Asp fl facilitated use of these primers for detection of A. fumigatus in clinical specimens of patients. The results were compared with microscopy, culture and serology. Application of PCR test to clinical samples of aspergillosis patients is discussed.

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