Bacterial and fungal counts in hospital air: comparative yields for 4 sieve impactor air samplers with 2 culture media


Gangneux JP, Robert-Gangneux F, Gicquel G, Tanquerel JJ, Chevrier S, Poisson M, AupÉe M, Guiguen C

Date: 22 June 2007


We compared the yields of 4 recently developed sieve impactor air samplers that meet international standard ISO 14698-1, using 2 growth media (tryptic soy agar and malt extract agar) in real conditions of use. Several hospital sites expected to have different densities of airborne microflora were selected in 2 hospitals. The Samplair MK2, Air Ideal, and Mas-100 samplers yielded higher bacterial counts than did the SAS Super-100 device (P<.05). No significant differences in fungal counts were noted between the 4 devices. The use of malt extract agar in addition to tryptic soy agar significantly improved the fungal yield.

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