Apparent mRNA instability in Aspergillus nidulans and Aspergillus terreus of a heterologous cDNA encoding the major capsid antigen of Rotavirus
Author:
Adela Villanueva, Andrew P. MacCabe, Javier Buesa and Daniel Ramón
Date: 4 April 2004
Abstract:
Two expression plasmids designed to produce the rotaviral VP6 protein inAspergillus nidulansand Aspergillus terreushave been constructed. In one ofthese plasmids the inducible A. terreusGla1 glucoamylase gene promoter andGla1 signal sequence are fused to the VP6 cDNA to enable induction and extracellularsecretion of the final protein product; in the other, the strong, constitutiveA. nidulans gpdAgene promoter has been employed. A. nidulans and A. terreustransformants containing intact copies of these plasmids have been obtained butneither intra- nor extra-cellular VP6 protein was detectable. Northern analysisindicated specific degradation of the VP6 mRNA. This lack of VP6 mRNA stabilitymay be related to fundamental differences between the general structure ofAspergillus mRNA and that of rotavirus, including codon usage and AU/GC ratio.
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