> Please forgive my ingnorance about Aspergillus. It would be appreciated if anybody help me with the following problems:
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> I have isolated an Aspergillus species from sorghum ergot infected heads, which produces white greenish colonies on PDA. It also produces yellowish metabolites in PDA and Potato Dextrose Broth. In PD Broth it also produces pellets. Now my questions are
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> 1. What is the probable name of the species
PDA is not a suitable medium for Aspergillus species from the environment. Media such as Czapek Yeast Extract agar with higher levels of carbohydrate are better. It is impossible to recognise an Aspergillus species from such a meagre description.
> 2. What is the name of yellowish metabolite produced
That of course depends on the species. But production of yellow metabolites by Aspergillus is very rare. Penicillium species produce yellow pigments much more frequently.
> 3. What medium/media suitable for maximum spore production of Aspergillus
Again it depends on the species. But CYA is usually effective.
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> Name of reference books/journals are appreciated.
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The best recent reference on general Aspergillus species is "Laboratory Guide to common Aspergillus species" by Maren A. Klich and J.I. Pitt. Published by CSIRO Division of Food Science, North Ryde, N.S.W. Australia. I sell this for US$44. See e-mail address.
Dr Latge is correct. Contact me and we will be happy to look at your isolate. Cost, about A$150.
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> Regards
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> S.A. Bhuiyan
> School of Land and Food
> The University of Queensland
> Queensland, Australia
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