A wide collection of laboratory procedures used in the preparation of aspergillus - DNA, RNA, protein and antigen detection. Procedures for handling clinical material, spore sampling and PCR detection of aspergillus are included.

 

 

Growth and Storage
Prepared by Dr Emma Prebble and Dr Michael Anderson

DNA & RNA Extraction
Prepared by Dr Emma Prebble and Dr Michael Anderson

Total lipid extraction
Prepared by Dr M. Birch

Strain identification
Prepared by Dr Michael Anderson

Protoplast preparation
Prepared by Dr Michael Birch, Dr Emma Prebble and Dr Michael Anderson

Protoplast preparation for A.nidulans
Prepared by Dr Raquel Lopez

Transformation
Prepared by Dr Christophe d'Enfert and Pieternella Mol

Protein/Antigen extraction
Prepared by Dr Nicola Sayers

In vitro killing assays
Prepared by Dr Nicola M. Sayers and Dr Cristina Gil Lamaignere

Measurement of serum drug level
Prepared by Dr Caroline Moore

Detection of fungi using fluorescent brighteners
Prepared by Dr Monod, M., Dr Baudraz-Rosselet, F., Dr Ramelet, A.A., and Dr Frenk, E

Sampling Aspergillus spores in air
Prepared by Dr Malcolm Richardson

Sampling Aspergillus spores in water
Prepared by Dr Adilia Warris MD PhD

Methods for assaying gliotoxin production in Aspergillus fumigatus
Prepared by Dr Richard Barton, Dr Louiza Belkacemi, Val Hopwood and E. Glyn V. Evans

Detection of medically important fungi using PCR
Prepared by Dr Jurgen Loeffler

A rapid method for generating gene deletions in Aspergillus fumigatus
Prepared by Dr Kim Langfelder, Stephanie Gattung, Prof. Axel A. Brakhage and Christophe d’Enfert

Eosinophilic fungal rhinosinusitis - Clinical material collection and culture technique
Prepared by Walter Buzina MD, Dr Hannes Braun & Prof. Heinz Stammberger

Microsatellite typing for Aspergillus fumigatus
Prepared by Alessandro Pasqualloto, Paul Bowyer, Jenny Bartholomew