Laboratory protocols

Displaying 1 - 25 of 366
Fungal Molecular Biology
RNA extraction: large scale prep
DNA extraction: miniprep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
Fungal ABC Transporter Deletion and Localization Analysis (Penicillium chrysogenum)
RNA extraction: Isolation of mRNA
A novel method of growing fungi for DNA extraction
PCR from spores
Isolation of RNA from yeast
DNA extraction – miniprep from spores for PCR
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
whole-cell screening
Susceptibility Testing Methods of Antifungal Agents
Model systems: in vivo
Animal Models to Evaluate Anti-infective Pharmacodynamics
Detection in clinical samples
Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Species/Strain identification
Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization
PCR ITS-RFLP for Penicillium Species and Other Genera
Secondary metabolites
Mycotoxins: A Fungal Genomics Perspective
PCR
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
Protein methods
Cell Wall Fractionation for Yeast and Fungal Proteomics
The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
Aflatoxins
Measurement of Aflatoxins Using Capillary Electrophoresis
DNA extraction
Isolation of Nucleic Acids for Fungal Diagnosis
Genetics
Multi-Copy Genetic Screen in Aspergillus nidulans

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