Laboratory protocols

Displaying 1 - 25 of 281
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Growth and Storage
A list of Aspergillus mutations and how to deal with them
Fungal Molecular Biology
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Two new easy to use vectors for transformations (FGSC)
A practical method for the preparation of total DNA from filamentous fungi
Improved Vectors for Selecting Resistance to Hygromycin
Preparation of chromosomal DNA in agarose plugs
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
RNA extraction: miniprep
Lambda IIBargem7B
RNA extraction: large scale prep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
RNA extraction: Isolation of mRNA
Species/Strain identification
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Adjuvant Properties of CpG Oligonucleotides in Primates
Secondary metabolites
Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
Planar liquid chromatography
Isolation by Planar Chromatography
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Model systems: in vivo
Murine Model of Invasive Aspergillosis
Evaluation of the Disease Liability of CFTR Variants