Laboratory protocols

Displaying 1 - 25 of 364
Fungal Molecular Biology
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
DNA Fingerprinting Candida Species
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Improved Vectors for Selecting Resistance to Hygromycin
A practical method for the preparation of total DNA from filamentous fungi
Three Decades of Fungal Transformation
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Preparation of chromosomal DNA in agarose plugs
Growth and Storage
Care and feeding of Aspergillus (FGSC)
A list of Aspergillus mutations and how to deal with them
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Cell Wall Fractionation for Yeast and Fungal Proteomics
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Species/Strain identification
PCR-RFLP for Aspergillus Species
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Model systems: in vivo
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Immunoinformatics as a Tool for New Antifungal Vaccines
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Genetics
Inducible Gene Expression Systems for Plants
Assaying mycotoxins
Immunochemical Method for Cyclopiazonic Acid

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