| Conference Proceedings |
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Fungal Genetics Conference 26th |
| Author |
Jonathan Palmer1,
Jeffery Theisen1, Rocio Duran2, Scott Grayburn2, Ana Calvo2, and Nancy Keller1. |
| Year |
2011 |
| Title |
The LaeA-like methyltransferase (LlmF) plays a role in secondary metabolism and development in Aspergillus nidulans. |
| Abstract |
The trimeric velvet complex, consisting of VelB/VeA/LaeA, has been shown to mediate development and secondary metabolism in response to light.
While exact functions have not been assigned to members of the complex, proper response to light is in part determined by the cellular location of VeA.
Briefly, under illumination VeA is localized mainly in the cytoplasm while in the absence of light VeA is exclusively nuclear. Here we describe a putative
methyltransferase LlmF (LaeA-Like Methyltransferase F) and report that it is a negative regulator of secondary metabolism and sexual development.
Strains lacking llmF produce increased levels of secondary metabolites in a velvet complex dependent fashion while over-expression of llmF represses
sexual development. We show that LlmF is required for proper cellular localization of VeA, possibly through direct protein-protein interactions as
LlmF-VeA interact in a yeast- two-hybrid assay. Further validation of the LlmF-VeA interaction is underway as well as biochemical characterization of
LlmF function. |
| Author Address |
1 University of Wisconsin, Madison, WI 53706 2 Northern Illinois
University, DeKalb, IL 60115 |
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